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prep cell 491 preparative electrophoresis apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad prep cell 491 preparative electrophoresis apparatus
    Prep Cell 491 Preparative Electrophoresis Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prep+cell+491+preparative+electrophoresis+apparatus/Model+491+Prep+Cell/pm34237624-70-8-14
    Average 90 stars, based on 1 article reviews
    prep cell 491 preparative electrophoresis apparatus - by Bioz Stars, 2026-10
    90/100 stars

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    Protein Purification:

    Article Title: Klebsiella pneumoniae enolase-like membrane protein interacts with human plasminogen.
    Article Snippet: Fractions showing enolase activity were concentrated and dialyzed by ultrafiltration in Amicon chromatography columns with 30 kDa membranes (Merck-Millipore, Burlington, MA, USA) into 20 mM Tris-HCl buffer pH 7.8 with 1 mM MgSO4. .. Preparative native protein purification was performed in a Prep Cell 491 preparative electrophoresis apparatus (Bio-Rad, Hercules, CA, USA) equipped with a tube of 37 mm in diameter. .. After purification on the DEAE Sephadex A-50 column, the specimen was dialyzed into 43 mM imidazole (Sigma-Aldrich, St. Louis, MO, USA) and 35 mM HEPES buffer (Sigma-Aldrich, St. Louis, MO, USA) at 4 ◦C and separated in a decreasing gradient of polyacrylamide separation gel (12 %, 10 %, 8%, 6%) and in thickening gel (4%).

    Electrophoresis:

    Article Title: Klebsiella pneumoniae enolase-like membrane protein interacts with human plasminogen.
    Article Snippet: Fractions showing enolase activity were concentrated and dialyzed by ultrafiltration in Amicon chromatography columns with 30 kDa membranes (Merck-Millipore, Burlington, MA, USA) into 20 mM Tris-HCl buffer pH 7.8 with 1 mM MgSO4. .. Preparative native protein purification was performed in a Prep Cell 491 preparative electrophoresis apparatus (Bio-Rad, Hercules, CA, USA) equipped with a tube of 37 mm in diameter. .. After purification on the DEAE Sephadex A-50 column, the specimen was dialyzed into 43 mM imidazole (Sigma-Aldrich, St. Louis, MO, USA) and 35 mM HEPES buffer (Sigma-Aldrich, St. Louis, MO, USA) at 4 ◦C and separated in a decreasing gradient of polyacrylamide separation gel (12 %, 10 %, 8%, 6%) and in thickening gel (4%).



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